文献名： Formulation and Characterization of Antithrombin Perfluorocarbon Nanoparticles
作者： Alexander J. Wilson1, Qingyu Zhou2, Ian Vargas1, Rohun Palekar3, Ryan Grabau1, Hua Pan1, Samuel A. Wickline1
1.The USF Health Heart InstituteUniversity of South FloridaTampaUSA
2.College of PharmacyUniversity of South FloridaTampaUSA
3.Department of Biomedical EngineeringWashington University in St. LouisSt. LouisUSA
摘要：Thrombin, a major protein involved in the clotting cascade by the conversion of inactive fibrinogen to fibrin, plays a crucial role in the development of thrombosis. Antithrombin nanoparticles enable site-specific anticoagulation without increasing bleeding risk. Here we outline the process of making and the characterization of bivalirudin and d-phenylalanyl-l-prolyl-l-arginyl-chloromethyl ketone (PPACK) nanoparticles. Additionally, the characterization of these nanoparticles, including particle size, zeta potential, and quantification of PPACK/bivalirudin loading, is also described.
关键词：Perfluorocarbon nanoparticles; Thrombin; PPACK; Bivalirudin; Anticoagulation
